Composite

Part:BBa_K3746011:Design

Designed by: Cheung Chi Ming   Group: iGEM21_Hong_Kong_JSS   (2021-10-19)


T7-LacO-DsbAss-FDRA-6His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 189
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The start codon of basic part FDR-A (BBa_K3746003) is removed in our designed DNA due to a N-terminal signal peptide (DsbAss) is added into this secretory construct.

Source

The sequence of MSMEG_5998 (coding sequence of FDR-A) was identified by a literature review [1] and the sequence was then extracted from NCBI (RefSeq: NZ_CP054795.1:4976460-4976939). Codon optimization for E. coli done by ThermoFisher Scientific GeneArt platform.

The other basic part sequences were identified from the iGEM part registry. The T7 promoter, LacO, RBS, and T7 terminator parts were used in the previous iGEM projects of our team.

We plan to synthesize this composite part by IDT in form of gene fragments and clone it into the pSB1C3 vector by restriction cloning.

References